Figure 2

Impact of cell density gradient on segregation in co-cultures. Upper panel: Time-sequence images showing the process of segregation in mixed co-cultures of C2C12 mouse myoblasts (red) and HaCaT human keratocytes (green). Left: A co-culture with cell density gradient evoking directional migration from the dense monolayer toward the cell-free space to the right. Right: A similar co-culture with homogeneous cell density. Lower panel: Growth of cluster sizes calculated from two-point correlation measurement. The curves and line colors correspond to co-cultures shown in the upper panel.