Figure 4

Imaging data for different time points in a segregation experiment with zebrafish ectoderm and mesoderm cells in culture. (a) Micro-molds are used to isolate small populations of ecto- and mesoderm cell mixtures labeled fluorescently with red and green nuclei, respectively. (b) Initial images show homogeneously mixed cells distributed throughout the mold. (c) Cells aggregate together on a time scale of roughly 100 minutes. (d) Imaging after sorting clearly shows the segregation of the two cell populations. Scale bar = 100 micron. From Klopper et al. (2010) with permission of Eur. Phys. J. E.